Search results for " molecular crowding"
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Printing Life-Inspired Subcellular Scale Compartments with Autonomous Molecularly Crowded Confinement.
2019
A simple, rapid, and highly controlled platform to prepare life-inspired subcellular scale compartments by inkjet printing has been developed. These compartments consist of fL-scale aqueous droplets (few µm in diameter) incorporating biologically relevant molecular entities with programmed composition and concentration. These droplets are ink-jetted in nL mineral oil drop arrays allowing for lab-on-chip studies by fluorescence microscopy and fluorescence life time imaging. Once formed, fL-droplets are stable for several hours, thus giving the possibility of readily analyze molecular reactions and their kinetics and to verify molecular behavior and intermolecular interactions. Here, this pla…
Bioanalysis in Femtoliter Scale Printed Artificial Systems
2020
Printing has fueled the development of a new class of artificial biosystems for the qualitative and quantitative determination of bioanalytes [1]. In particular, the multiscale organization (from nanometers to millimeters) and multiplexed molecular composition (DNA, proteins, lipids, polymers) of such platforms enable the determination of molecular interactions in conditions mimicking/redesigning those of the living systems. Since the development of microarrays platforms [2], the downsizing of the “analyzable” feature down to femtoliter (fL) scales has permitted to develop new researches in the field of molecular condensates/confinement. Herein, two relevant examples of fL-scale systems wil…